Background Essay: Recombinant DNA Lab in the Classroom and in Real-World Research

Lab experiments like the one featured in this video provide students with hands-on experience using common biotechnology research tools and techniques. For students, this lab allows them to connect what they learn in the classroom to real science problems. In addition to providing a high degree of authenticity, these labs can serve to reinforce the importance of precision and collaboration in successful experimentation.

In this video, students are shown using micropipettes—precision instruments used to transfer very small but specific volumes of liquid from one vessel to another—in order to deliver DNA plasmid into a microfuge tube containing bacteria. All micropipettes are designed to be reliable and precise. Lab technicians and scientists must practice with them to deliver just the right volume of material in just the right way, as even small errors can be costly.

Successful experimentation in a high school lab, as in a professional research facility, relies on collaboration. It’s important that team members know which tasks have been assigned to each person and that members frequently communicate with one another. Failure to do so can lead to mistakes that can jeopardize an experiment or any other research objective.

Errors caused by poor lab technique or poor communication can contaminate samples, invalidate data, and waste precious materials and time. Having to repeat research experiments can be both time-consuming and expensive. The more experience students can get in a real-world setting the better prepared they’ll be for a career in science.