Discussion Questions: Using a Micropipette

• Describe two techniques that can help avoid sample contamination when using micropipettes.
• Why is it important not to use micropipettes beyond their volume limit?
• What are the roles of the first and second stops on a micropipette?
• If the window of a micropipette reads 175, how many microliters would this reading represent on a P20 micropipette? How many microliters would it represent on a P200 micropipette?